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Molecular Formula: C8H10N4O4 Formula Weight: 226.19 Synonyms :
Download CoA for Azidobutyrate NHS Ester.
Coupling: For labeling 0.2 umoles of an amino-modified oligonucleotide, dissolve 2.3 mg of Azidobutyrate NHS Ester in 60 uL of ACN (DMSO or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500 uL of 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12 uL of the Azidobutyrate NHS Ester solution to the dissolved oligonucleotide and let react for 2 hours at room temperature and desalt.Storage: dry, -10 to -30°CDiluent: anhydrous ACN, DMSO or DMFStability in Solution: 2-3 days
Quality Control: NMR: 95+ % TLC: pure
In this case, oligonucleotides are synthesized as usual on any synthesizer. Amino groups have to be introduced either at the 3’ or at the 5’ end of the oligonucleotide. After cleavage and deprotection, the azido group can be introduced as a modification of the amine. For example, the oligonucleotide in sodium carbonate/sodium bicarbonate buffer (pH 8.75) was incubated at room temperature with succinimidyl-4-azidobutyrate in DMSO. The final oligo is then purified using HPLC or precipitated with ethanol or butanol.
REFERENCES:J. Am. Chem. Soc., 2007, 129 (21), pp 6859–6864 DOI: 10.1021/ja070273v Template-Directed Oligonucleotide Strand Ligation, Covalent Intramolecular DNA Circularization and Catenation Using Click ChemistryLangmuir, 2010, 26 (11), pp 8666–8672 DOI: 10.1021/la904822fCovalent Attachment of Lipid Vesicles to a Fluid-Supported Bilayer Allows Observation of DNA-Mediated Vesicle Interactions
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